|
|
|
| Registros recuperados: 43 | |
|
| |
|
|
| Amaral,Alexandre Morais do; Saito,Daniel; Formighieri,Eduardo Fernandes; Rabello,Edenilson; Souza,Adriane N. de; Silva-Stenico,Maria Estela; Tsai,Siu Mui. |
| Pleiotropic drug resistance (PDR) proteins, a subfamily of the ATP-binding cassette (ABC) transporters, have been recently shown to play a role in plant defense against biotic and abiotic stresses. However, nothing is known about their expression in citrus. To investigate the occurrence of PDR homologues in citrus species, we have surveyed EST sequences from different tissues and conditions of the Citrus Expressed Sequence Tags (CitEST) database, through sequence similarity search analyses and inspections for characteristic PDR domains. Multiple sequence alignments, prediction of transmembrane topology and phylogenetic analysis of PDR-like proteins were additionally performed. This study allowed the identification of nine putative proteins showing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ABC transporters; CitEST; Expressed sequence tag; Transcriptome; Stress. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500014 |
| |
|
|
| Barbosa,Aulus Estevão; Marinho,Paulo. |
| The Eucalyptus Genome Sequencing Project (FORESTs), an initiative from the Brazilian ONSA consortium (Organization for Nucleotide Sequencing and Analysis), has achieved the sequencing of 123.889 EST clones from 18 different cDNA libraries. We have investigated the FORESTs data set to identify EST clusters potentially encoding thioredoxins (TRX). Two types of thioredoxin families described in plants, chloroplastic (TRXm/f/x/y) and cytosolic (TRXh), have been found in the transcriptome. Putative typical TRXs have been identified in fifteen clusters, four m-type, seven h-type, two f-type, one cluster for each x/y-types and one putative homologue of the TDX gene from Arabidopsis thaliana. One cluster presents an atypical active site WCMPS, different from the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eucalyptus; Transcriptome; Thioredoxin. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400008 |
| |
|
|
| Benzekri, Hicham; Armesto, Paula; Cousin, Xavier; Rovira, Mireia; Crespo, Diego; Alejandro Merlo, Manuel; Mazurais, David; Bautista, Rocio; Guerrero-fernandez, Dario; Fernandez-pozo, Noe; Ponce, Marian; Infante, Carlos; Zambonino, Jose Luis; Nidelet, Sabine; Gut, Marta; Rebordinos, Laureana; Planas, Josep V.; Begout, Marie-laure; Gonzalo Claros, M.; Manchado, Manuel. |
| Background Senegalese sole (Solea senegalensis) and common sole (S. solea) are two economically and evolutionary important flatfish species both in fisheries and aquaculture. Although some genomic resources and tools were recently described in these species, further sequencing efforts are required to establish a complete transcriptome, and to identify new molecular markers. Moreover, the comparative analysis of transcriptomes will be useful to understand flatfish evolution. Results A comprehensive characterization of the transcriptome for each species was carried out using a large set of Illumina data (more than 1,800 millions reads for each sole species) and 454 reads (more than 5 millions reads only in S. senegalensis), providing coverages ranging from... |
| Tipo: Text |
Palavras-chave: Soles; Transcriptome; Microarray; Orthology; Molecular markers; SoleaDB. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00238/34918/33626.pdf |
| |
|
| |
|
| |
|
|
| Dubrulle, Guillaume; Picot, Adeline; Madec, Stephanie; Corre, Erwan; Pawtowski, Audrey; Baroncelli, Riccardo; Zivy, Michel; Balliau, Thierry; Le Floch, Gaétan; Pensec, Flora. |
| The fungal phytopathogen Colletotrichum lupini is responsible for lupin anthracnose, resulting in significant yield losses worldwide. The molecular mechanisms underlying this infectious process are yet to be elucidated. This study proposes to evaluate C. lupini gene expression and protein synthesis during lupin infection, using, respectively, an RNAseq-based transcriptomic approach and a mass spectrometry-based proteomic approach. Patterns of differentially-expressed genes in planta were evaluated from 24 to 84 hours post-inoculation, and compared to in vitro cultures. A total of 897 differentially-expressed genes were identified from C. lupini during interaction with white lupin, of which 520 genes were predicted to have a putative function, including... |
| Tipo: Text |
Palavras-chave: Anthracnose disease; Transcriptome; Proteome; Pathogenicity factors. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00656/76795/77961.pdf |
| |
|
|
| Ellison, Amy R.; Uren Webster, Tamsyn M.; Rey, Olivier; Garcia De Leaniz, Carlos; Consuegra, Sofia; Orozco-terwengel, Pablo; Cable, Jo. |
| Background: Captive animal populations, be it for food production or conservation programmes, are often maintained at densities far beyond those in natural environments, which can have profound effects on behaviour, immune and stress levels, and ultimately welfare. How such alterations impact transcriptional responses to pathogen infection is a 'different kettle of fish' and remains poorly understood. Here, we assessed survival and gene expression profiles of infected fish reared at two different densities to elucidate potential functional genomic mechanisms for density-related differences in disease susceptibility. Results: Utilising a whole-transcriptome sequencing (RNAseq) approach, we demonstrate that rearing density in tilapia (Oreochromis niloticus)... |
| Tipo: Text |
Palavras-chave: Stocking density; Stress; Saprolegnia; Aquaculture; Transcriptome; Tilapia; Circadian rhythm; Disease risk. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00462/57353/78952.pdf |
| |
|
|
| Fassio, Giulia; Modica, Maria Vittoria; Mary, Lou; Zaharias, Paul; Fedosov, Alexander E.; Gorson, Juliette; Kantor, Yuri I.; Holford, Mandё; Puillandre, Nicolas. |
| Profundiconus is the most divergent cone snail genus and its unique phylogenetic position, sister to the rest of the family Conidae, makes it a key taxon for examining venom evolution and diversity. Venom gland and foot transcriptomes of Profundiconus cf. vaubani and Profundiconus neocaledonicus were de novo assembled, annotated, and analyzed for differential expression. One hundred and thirty-seven venom components were identified from P. cf. vaubani and 82 from P. neocaledonicus, with only four shared by both species. The majority of the transcript diversity was composed of putative peptides, including conotoxins, profunditoxins, turripeptides, insulin, and prohormone-4. However, there were also a significant percentage of other putative venom components... |
| Tipo: Text |
Palavras-chave: Conidae; Conotoxins; Turripeptides; Transcriptome; Venom gland. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00589/70155/68142.pdf |
| |
|
|
| Foulon, Valentin; Boudry, Pierre; Artigaud, Sebastien; Guérard, Fabienne; Hellio, Claire. |
| Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Pacific oyster; Pediveliger larvae; Bioadhesive; Transcriptome. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00475/58677/61172.pdf |
| |
|
|
| Fu,Suhong; Lei,Ming; Zhang,Yongqun; Deng,Zhaomin; Shi,Jing; Hao,Doudou. |
| Abstract Dysphania schraderiana is widely distributed in Lhasa (Tibet, China) and used as a traditional medicine. However, the lack of genetic information hinders the understanding of its physiological processes, such as the biosynthesis of secondary metabolites. Herein, we used Illumina Hiseq4000 platform to sequence the transcriptome of flower and leaf tissues from D. schraderiana for the first time. Totally, 40,142 unigenes were assembled from approximately 5.2 million clean reads. All unigenes underwent gene prediction and were subsequently annotated in a NR (NCBI non-redundant protein) database, COG (Clusters of Orthologous Groups of proteins) database, and KEGG (Kyoto Encyclopedia of Genes and Genomes) database. Among the 40,142 unigenes, 2,579 genes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dysphania schraderiana; De novo assembly; Transcriptome; Annotation; Terpenoid biosynthesis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300480 |
| |
|
|
| Harney, Ewan; Lachambre, Sebastien; Roussel, Sabine; Huchette, Sylvain; Enez, Florian; Morvezen, Romain; Haffray, Pierrick; Boudry, Pierre. |
| Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by... |
| Tipo: Text |
Palavras-chave: Abalone; Parentage assignment; Pedigree; Selective breeding; Transcriptome; SNP. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00429/54061/55396.pdf |
| |
|
|
| He,Ruishi; Zhu,Xinxin; Li,Qiaoyun; Jiang,Yumei; Yu,Dongyan; Sun,Yulong; Liang,Xiaolong; Ni,Yongjing; Niu,Jishan. |
| ABSTRACT: Wheat (Triticum aestivum L.) stem development significantly affects grain yield. The dwarf plants (D) of wheat mutant dms was less than 30cm. Here, we were to explore the molecular basis for the restrained stem development of the dwarf plants. The results were reached by compare the young spikes and stems transcriptomes of the tall (T) and D plants of mutant dms. We identified 663 genes highly expressed in stem tips. We identified 997 differentially expressed genes (DEGs) in stem tips between T and D, 403 DEGs were significantly related with stem development. Most biological processes in stem tips on dwarf plants were significantly suppressed, such as phytohormone signaling etc. The sequencing analysis results were confirmed by quantitatively... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Wheat (Triticum aestivum L.); Dms mutant; Transcriptome; Stem development.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200401 |
| |
|
|
| Hindmarch,C.C.T.; Franses,P.; Goodwin,B.; Murphy,D.. |
| The supraoptic nucleus (SON) is part of the central osmotic circuitry that synthesises the hormone vasopressin (Avp) and transports it to terminals in the posterior lobe of the pituitary. Following osmotic stress such as dehydration, this tissue undergoes morphological, electrical and transcriptional changes to facilitate the appropriate regulation and release of Avp into the circulation where it conserves water at the level of the kidney. Here, the organisation of the whole transcriptome following dehydration is modelled to fit Zipf's law, a natural power law that holds true for all natural languages, that states if the frequency of word usage is plotted against its rank, then the log linear regression of this is -1. We have applied this model to our... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transcriptome; Supraoptic nucleus; Dehydration; Zipf; Microarray; Vasopressin. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201000 |
| |
|
| |
|
| |
|
| |
|
| |
|
|
| Ma,Juan; Gao,Bo; Wang,Rongyan; Li,Xiuhua; Chen,Shulong. |
| Abstract The objective of this study was to identify molecular responses in Ditylenchus destructor to cold and desiccation by means of transcriptomes analyses. A total of 102,517 unigenes were obtained, with an average length of 1,076 bp, in which 58,453 (57%) had a functional annotation. A total of 1154 simple sequence repeats (SSRs) distributed over 1078 unigenes were detected. Gene expression profiles in response to cold and desiccation stress and the expression of specific stress-related genes were compared. Gene ontology analysis and pathway-based analysis were used to further investigate the functions of the differentially expressed genes. The reliability of the sequencing data was verified through quantitative real-time PCR analysis of 19... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Potato rot nematode; Transcriptome; Tolerance; Differentially expressed genes. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100202 |
| |
|
| |
| Registros recuperados: 43 | |
|
|
|
